Seaway Trail follows the shoreline of Lake Erie and Lake Ontario as it travels from southwest New York to Rooseveltown. The byway is 454 miles long and follows a series of two-lane paved roads that are suitable for all types of vehicles. For detailed directions, please refer to "Following the Seaway Trail" below. The byway is open year-round.

Seaway Trail travels along the shores of New York's Great Lakes, the Niagara River, and Saint Lawrence River, connecting quaint villages, historic sites, picturesque bays, and rolling farmland. The route is part of the National Park Service's National Recreational Trail system and is the longest such trail in the United States. Several scenic overlooks offer spectacular views of the Great Lakes.